FastQCFastQC Report
Tue 3 Jul 2018
HWLKJBGX5_n01_mycscreens2_5_bc_bc20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLKJBGX5_n01_mycscreens2_5_bc_bc20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16963020
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGC10940196.449435301025407No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGC9079715.352649469257243No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGCGG8848645.216429621612189No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGCG8812255.194977073657875No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCGCGCGGCG8694945.125820755973877No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAATACGGACCGGA1570290.9257136995652896No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAATACGGACCGGATT1341810.7910207026814801No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAATACGGA1328580.7832213839280977No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAATACGG1282710.7561802084770283No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAATACGGACCGGAT1262820.7444547020518751No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGGTAGCGCACGATATT968480.5709360715249997No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCTTTGGGGATCTCAA946210.5578075130489736No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGTAGCGCACGATATTAG827780.48799093557633016No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGTAGCGCACG826780.4874014179078961No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCTTTGGGGATCTCAAGA816100.48110536920902053No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCTTTGGGGAT804900.4745027713225593No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGTAGCGCAC796110.4693209110170241No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGGTAGCGCACGATATTA784570.46251787712329523No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCTTTGGGGA777830.4585445280380498No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCTTTGGGGATCTCAAG770180.45403471787452937No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGT732260.4316802078875106No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTC617840.36422759626528767No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGTCA614640.3623411397262987No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCTGAAGGTT595350.3509693439022061No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGTC592790.349460178671015No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCT396130.23352563399677653No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGGATTCTGAGGGAAAT389010.22932826819752616No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGGCCGCCACTGGAGGG341750.2014676631873334No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGAGACGGACG330480.19482379906408173No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGATTCTGAGG328530.19367423961063537No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT325780.19205306602244177No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGATTCTGAGGGAAATTG323680.19081507891873026No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTG317000.1868771008935909No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGATTCTGAG316720.18671203594642935No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGAGACGGAC316020.18629937357852552No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGGATTCTGAGGGAAATT306900.18092297244240707No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGCCGCCACTG286730.1690324010700925No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGCCGCCACTGGAGGGCG277530.16360883852049932No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGGCCGCCACTGGAGGGC276540.1630252160287496No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGCCGCCACT275830.16260665848416145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG3138150.069.9610061
TAACAAT3223050.069.838241
TACTGTA3130400.069.772932
TAGGTCG3920850.069.768023
ATAGGTC3923600.069.7617342
AGGTCGC3922200.069.707624
TCGATAC3307350.069.6703644
ACTGTAT3135000.069.657353
TCGATAG3155100.069.640145
CTGTATC3135350.069.639734
GGTCGCA3926000.069.623765
ATACTGT3137650.069.6218951
GTATCTC3136550.069.5890666
TGTATCT3139000.069.5724645
GTCGCAT3929850.069.56116
TCGCATC3930150.069.547297
TATCTCT3141950.069.538957
AACAATG3250850.069.5230942
ATCTCTT3143500.069.445248
CGATAGG3161100.069.4051746
Produced by FastQC (version 0.11.2)