FastQCFastQC Report
Tue 3 Jul 2018
HWLKJBGX5_n01_mycscreens2_5_bc_bc18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLKJBGX5_n01_mycscreens2_5_bc_bc18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12201819
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGC139920.11467142726834416No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCGCGCGGCG122490.10038667185605686No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGC122490.10038667185605686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG2154850.069.9504241
TAGGTCG2716500.069.768523
TAACAAT2374000.069.7529451
TCGATAC2417200.069.74314
AGGTCGC2716900.069.724044
ATAGGTC2719900.069.6813052
GGTCGCA2719300.069.6358955
TACTGTA2371350.069.601742
GTCGCAT2722500.069.573336
TCGATAG2168500.069.5698245
TCGCATC2723050.069.5565647
CTGTATC2372400.069.531674
ACTGTAT2373550.069.507733
GTATCTC2375050.069.4277956
TGTATCT2377300.069.418035
TATCTCT2380250.069.343437
AACAATG2395850.069.3428652
ATACTGT2381300.069.340691
ATCTCTT2380800.069.232988
AATGGTC2397300.069.217065
Produced by FastQC (version 0.11.2)