FastQCFastQC Report
Tue 3 Jul 2018
HWLKJBGX5_n01_mycscreens2_5_bc_bc17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLKJBGX5_n01_mycscreens2_5_bc_bc17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11976959
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGC298650.249353780037153No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGCG274530.22921511211652307No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGCGG270630.22595885984079933No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCGCGCGGCG269850.22530760938565458No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGC231450.19324604851698998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG2323650.069.946681
TAACAAT2467250.069.796541
TAGGTCG2634300.069.758563
AGGTCGC2633950.069.728834
ATAGGTC2639950.069.684842
TCGATAC1924350.069.668754
GGTCGCA2637300.069.639065
TACTGTA2462450.069.6362152
TCGATAG2336400.069.626175
CTGTATC2462800.069.5774464
GTCGCAT2640050.069.577136
ACTGTAT2464250.069.565463
TCGCATC2639950.069.556187
TGTATCT2468300.069.4522255
GTATCTC2466850.069.449056
AACAATG2488650.069.413652
ATACTGT2471650.069.372091
TATCTCT2472600.069.360047
ATCTCTT2471000.069.337838
GCATCTT2650650.069.2713559
Produced by FastQC (version 0.11.2)