FastQCFastQC Report
Tue 3 Jul 2018
HWLKJBGX5_n01_mycscreens2_5_bc_bc15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLKJBGX5_n01_mycscreens2_5_bc_bc15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10005034
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGC109377010.932196732165028No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGCG106976210.692237527628592No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCGCGCGGCG101727610.167641609213922No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGC101454510.140345350150733No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGCGG9367689.362966682571994No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAATACGGACCGGA1226251.2256330163395746No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAATACGGACCGGAT1211361.2107505081941752No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAATACGGA1165641.165053512062028No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAATACGG1157761.1571774768581495No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAATACGGACCGGATT1112841.1122800782086297No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGGTAGCGCACGATATT705890.7055348337646828No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGGTAGCGCACGATATTA697910.6975588488754761No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGTAGCGCACG665890.6655549596333206No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGTAGCGCAC664510.6641756539757886No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGTAGCGCACGATATTAG644940.6446155005570195No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCTTTGGGGATCTCAA638940.6386185194373153No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCTTTGGGGATCTCAAG626110.6257949748096808No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCTTTGGGGA616000.615690061622979No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCTTTGGGGAT611500.6111923257832007No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCTTTGGGGATCTCAAGA577260.5769695535267546No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGT395500.39530100547384445No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGTC385710.3855159312801935No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCTGAAGGTT372590.3724025325651067No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTC371090.3709032872851807No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGTCA357270.357090240772795No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCT314000.3138420119311938No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTG312190.3120329226267497No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGAGACGGAC299490.29933931259004215No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGAGACGGACG296310.2961609125965989No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT275360.2752214535202979No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGGATTCTGAGGGAAAT237600.2374804523402919No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGATTCTGAG227960.22784530267463357No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGGATTCTGAGGGAAATT227780.22766539324104246No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGATTCTGAGG225960.22584630896806548No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGATTCTGAGGGAAATTG212510.21240307629139493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAAC455.456968E-1270.0079967
TCGCGTC600.070.007997
TTTTTGT252.8427767E-669.99924516
ACGTTCG1150.069.998891
ACGATCG1976500.069.963481
TAACAAT1825800.069.9068761
ATAGGTC2082800.069.8367842
ATACTGT2033400.069.831941
TACTGTA2032950.069.817172
TAGGTCG2083050.069.788083
TCGATAC1943600.069.744064
AGGTCGC2083600.069.7364044
ACTGTAT2034000.069.732953
CTGTATC2034300.069.702364
TCGATAG1985400.069.675145
AACAATG1837700.069.667722
GGTCGCA2084800.069.650655
TGTATCT2038200.069.623625
GTATCTC2037700.069.601546
GTCGCAT2088500.069.584596
Produced by FastQC (version 0.11.2)