FastQCFastQC Report
Tue 3 Jul 2018
HWLKJBGX5_n01_mycscreens2_5_bc_bc14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLKJBGX5_n01_mycscreens2_5_bc_bc14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12240785
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGC908590.7422644871223537No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGCG806120.6585525356421177No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGCGG788970.6445419962853689No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCGCGCGGCG787040.6429653000195658No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGC780030.6372385431163116No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGT378860.30950629391824136No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGTC353670.2889275483557631No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGTCA349340.2853901935210854No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTC345510.28226130922159No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCTGAAGGTT340040.2777926415666969No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAATACGGACCGGA173250.14153504044062534No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAATACGGACCGGATT162660.13288363450546678No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAATACGGACCGGAT161350.13181344170328946No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAATACGGA155330.126895456459696No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAATACGG152830.12485310378378511No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG2316400.069.957881
TAACAAT2395750.069.7883451
TAGGTCG2616550.069.773053
TCGATAC2339950.069.7344
AGGTCGC2616250.069.7251364
ATAGGTC2621200.069.693342
TCGATAG2329100.069.653095
TACTGTA2406700.069.648152
GGTCGCA2619100.069.641245
CTGTATC2408050.069.602124
GTCGCAT2621700.069.592216
ACTGTAT2409400.069.565743
TCGCATC2621900.069.563447
TGTATCT2413100.069.449215
ATACTGT2413800.069.428881
GTATCTC2412700.069.418656
AACAATG2419300.069.333162
TATCTCT2417950.069.3321847
AATGGTC2419500.069.2712865
GCATCTT2631650.069.266049
Produced by FastQC (version 0.11.2)