FastQCFastQC Report
Tue 3 Jul 2018
HWLKJBGX5_n01_mycscreens2_5_bc_bc13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLKJBGX5_n01_mycscreens2_5_bc_bc13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8241753
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGCGG107310313.020324680926498No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGC93749611.3749587011404No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCGCGCGGCG8143059.880240283832821No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGCGGCGCG7951009.647219468964915No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCGCGCGGCGC7429169.014053199604502No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAATACGGACCGGATT1644801.995691935926738No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAATACGGACCGGA1353791.642599578026665No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAATACGG1205201.4623102633626608No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAATACGGACCGGAT1153851.4000055570702008No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAATACGGA966951.1732334128431172No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGTAGCGCACGATATTAG961931.1671424756359479No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCTTTGGGGATCTCAAGA870361.056037471639832No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGGTAGCGCACGATATT795490.9651951471974469No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCTTTGGGGATCTCAA715020.8675581517669845No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGTAGCGCAC703930.8541022765423811No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGGTAGCGCACGATATTA678270.8229681234077265No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCTTTGGGGA644890.782467030982365No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCTTTGGGGATCTCAAG612630.7433248727546191No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGTAGCGCACG573650.6960291093411802No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGTCA523420.6350833372463358No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCTTTGGGGAT510590.6195162606790084No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGT436320.5294019366996318No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGT426240.5171715289210924No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCTGAAGGTT388460.4713317664336701No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTCCAGGTC372590.45207615418710073No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCT366490.4446748161465164No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGATTCTGAGGGAAATTG365700.44371628220355547No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGAGACGGAC327890.3978401196929828No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCTGAAGGTTC316970.38459051126623184No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTG313730.380659308765987No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGGATTCTGAGGGAAAT306920.37239650351084286No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGAGACGGACG285110.3459336866804914No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGATTCTGAG274120.3325991448663895No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGGATTCTGAGGGAAATT253680.3077985957599069No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGATTCTGAGG220780.26787990370495207No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCTCCGCGCTTCTGCTTC130660.15853423416110626No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCTCCGCGCTTCTGCT112180.1361118199004508No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCTCCGCGCT98440.11944060929756084No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCTCCGCGCTTCTGCTT95730.11615247387297338No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCTCCGCGCTT85520.10376433266078224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA401.3460522E-1070.005277
TCGATCT550.070.005277
TCGCGTC550.070.005277
ACGGCGT150.00222488970.00449
CATTTTG301.0247095E-770.0035710
GTCGAAT252.8418945E-670.002736
TTCGCAT353.7161954E-970.002726
GGTTTTC700.070.002312
TCGTTAC700.070.001454
ACGCTCG353.7180143E-969.999331
ACGGTCG353.7180143E-969.999331
AACGCGA150.002225531369.998930
TACGACC207.932371E-569.99847441
ACGATCG1533400.069.971941
TAACAAT2041050.069.913581
TACTGTA1479850.069.86192
ATACTGT1480150.069.845631
ACTGTAT1480450.069.809953
ATAGGTC1726000.069.806782
TAGGTCG1725700.069.774293
Produced by FastQC (version 0.11.2)