FastQCFastQC Report
Tue 3 Jul 2018
HWLKJBGX5_n01_mycscreens2_5_bc_bc12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLKJBGX5_n01_mycscreens2_5_bc_bc12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12184308
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTCG455.456968E-1269.999431
ACGATCG2518550.069.957731
TCGATTC2556000.069.849222
ATCGATT2556450.069.835141
TAACAAT2384600.069.780731
TCGATAA2433750.069.690793
TCGATAG2533800.069.61785
GATAACG2432250.069.6174855
TAACGCA2430850.069.590767
ATTCCTT2561550.069.590565
ATAACGC2432450.069.5772256
TCGATAC2145950.069.568814
CGATTCC2563000.069.538553
AACGCAT2433250.069.520118
CGATAAC2438600.069.503374
ACGCATT2434400.069.4760749
TTGGTTC2560600.069.3814410
AACAATG2406450.069.368432
GATTCCT2571750.069.3496554
TTCCTTG2570350.069.322356
Produced by FastQC (version 0.11.2)