FastQCFastQC Report
Tue 3 Jul 2018
HWLKJBGX5_n01_mycscreens2_5_bc_bc11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLKJBGX5_n01_mycscreens2_5_bc_bc11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9876623
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTTAG150.002225098770.0024448
ACGGTCG455.456968E-1269.9992451
ACGATCG1865950.069.961731
TCGATTC1580100.069.853932
TCGATAA2822750.069.80123
TAACAAT1835550.069.785691
ATCGATT1581700.069.760261
GATAACG2822350.069.711875
TAACGCA2819950.069.6832357
ATAACGC2821950.069.682066
CGATAAC2826300.069.672834
TCGATAG1875450.069.644965
AACGCAT2822050.069.607118
TCGATAC1655850.069.591654
ACGCATT2824650.069.5529869
ATTCCTT1585000.069.538865
CGATTCC1588850.069.36793
AACAATG1855750.069.312342
CGCATTT2831450.069.3084210
TTGGTTC1583050.069.2934110
Produced by FastQC (version 0.11.2)