FastQCFastQC Report
Tue 3 Jul 2018
HWLKJBGX5_n01_mycscreens2_5_bc_bc10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLKJBGX5_n01_mycscreens2_5_bc_bc10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14325078
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG2416150.069.9553451
TCGATTC3260700.069.829872
ATCGATT3261000.069.818861
TCGATAA3836150.069.8003853
TAACAAT2465400.069.726261
ATAACGC3835050.069.677736
GATAACG3837850.069.671585
TAACGCA3834200.069.651187
CGATAAC3843850.069.61944
ATTCCTT3266550.069.608915
TCGATAG2431850.069.605255
AACGCAT3837400.069.590118
TCGATAC2181350.069.581664
ACGCATT3840000.069.54139
CGATTCC3269900.069.539453
GATTCCT3278750.069.395184
TTGGTTC3266850.069.391410
TTCCTTG3276850.069.375156
GGTTCTT3267550.069.3105812
AACAATG2489350.069.299672
Produced by FastQC (version 0.11.2)