FastQCFastQC Report
Wed 20 Sep 2023
HWLGMBGXT_n01_AtbZIP72_ampCol-B_ae2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLGMBGXT_n01_AtbZIP72_ampCol-B_ae2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6301968
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCGGGGGTGGG177660.28191193608091947TruSeq Adapter, Index 8 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCGGGGGGGGG168910.2680273844614888TruSeq Adapter, Index 8 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCGGGGGTGGC147920.23472032863384898TruSeq Adapter, Index 8 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCGGGGTTGGC82230.13048304910466066TruSeq Adapter, Index 8 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCGGGGGTTGG63130.10017505642681777TruSeq Adapter, Index 8 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)