FastQCFastQC Report
Wed 20 Sep 2023
HWLGMBGXT_n01_AtbZIP72_ampCol-B_ae1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLGMBGXT_n01_AtbZIP72_ampCol-B_ae1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6105461
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATGGGGGTTTGC113890.1865379207237586TruSeq Adapter, Index 13 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATGGGGGGTTGC97600.15985688877547494TruSeq Adapter, Index 13 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATGGGGGGTGGG94430.15466481564618953TruSeq Adapter, Index 13 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATGGGGGGTGGC94270.15440275517278712TruSeq Adapter, Index 13 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATGGGGGTTGGC94040.15402604324227115TruSeq Adapter, Index 13 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATGGGGGGTTGG91940.1505864995288644TruSeq Adapter, Index 13 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCGGGGTTTGC84020.13761450609544473TruSeq Adapter, Index 13 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCGGGGTTGGC78550.12865531366099955TruSeq Adapter, Index 13 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)