FastQCFastQC Report
Wed 20 Sep 2023
HWLGMBGXT_n01_AtbZIP64_ampCol-B_ae2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLGMBGXT_n01_AtbZIP64_ampCol-B_ae2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8332146
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATGGGGGGGGGG322780.3873911954975345TruSeq Adapter, Index 6 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATGGGGGGTGGG173570.2083136805332024TruSeq Adapter, Index 6 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATGGGGGGTTGG105740.1269060815785033TruSeq Adapter, Index 6 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATGGGGGGGTGG100730.12089322486667901TruSeq Adapter, Index 6 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)