FastQCFastQC Report
Wed 20 Sep 2023
HWLGMBGXT_n01_AtbZIP56_ampCol-B_ae2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLGMBGXT_n01_AtbZIP56_ampCol-B_ae2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3940983
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATGCC1933464.906034864905533TruSeq Adapter, Index 7 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT173890.4412350928689619TruSeq Adapter, Index 7 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGAGCTCGTATGCC127610.32380246248207617TruSeq Adapter, Index 7 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATGTCGTATGCC83170.21103871800512716TruSeq Adapter, Index 7 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGCTCTCGTATGCC75670.1920079330461461TruSeq Adapter, Index 7 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATGGC73640.18685693391724856TruSeq Adapter, Index 7 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTTTGCC51670.13110942117740676TruSeq Adapter, Index 7 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATTCC50330.12770925426473545TruSeq Adapter, Index 7 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)