FastQCFastQC Report
Wed 20 Sep 2023
HWLGMBGXT_n01_AtbZIP56_ampCol-B_ae1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLGMBGXT_n01_AtbZIP56_ampCol-B_ae1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7015906
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCC1318681.8795576793645754TruSeq Adapter, Index 2 (97% over 34bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGGC192500.2743765381121127TruSeq Adapter, Index 2 (97% over 34bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTTTGCC161780.23059031862741602TruSeq Adapter, Index 2 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT134770.1920920833317892TruSeq Adapter, Index 2 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTTTGGC105880.15091422262498955TruSeq Adapter, Index 2 (97% over 34bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATTCC97920.13956857460747052TruSeq Adapter, Index 2 (97% over 34bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCGCGTATGCC95000.13540660322415948TruSeq Adapter, Index 2 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)