FastQCFastQC Report
Wed 20 Sep 2023
HWLGMBGXT_n01_AtbZIP55_ampCol-B_ae2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLGMBGXT_n01_AtbZIP55_ampCol-B_ae2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7038074
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATGCC2073182.945663827916558TruSeq Adapter, Index 7 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT193880.27547309107576873TruSeq Adapter, Index 7 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGAGCTCGTATGCC139050.1975682551789026TruSeq Adapter, Index 7 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATGTCGTATGCC90450.12851527278627647TruSeq Adapter, Index 7 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGCTCTCGTATGCC80740.11471888474034232TruSeq Adapter, Index 7 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATGGC78280.11122361032293779TruSeq Adapter, Index 7 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)