FastQCFastQC Report
Wed 20 Sep 2023
HWLGMBGXT_n01_AtbZIP55_ampCol-B_ae1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLGMBGXT_n01_AtbZIP55_ampCol-B_ae1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7054895
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCC1375661.9499368877920933TruSeq Adapter, Index 2 (97% over 34bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGGC200940.28482351615438645TruSeq Adapter, Index 2 (97% over 34bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTTTGCC168720.23915309866411905TruSeq Adapter, Index 2 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT146270.2073312217970643TruSeq Adapter, Index 2 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTTTGGC108690.15406324261381638TruSeq Adapter, Index 2 (97% over 34bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATTCC103750.1470610122475246TruSeq Adapter, Index 2 (97% over 34bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCGCGTATGCC100480.14242593263259057TruSeq Adapter, Index 2 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)