FastQCFastQC Report
Wed 20 Sep 2023
HWLGMBGXT_n01_AtbZIP47_ampCol-B_ae1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLGMBGXT_n01_AtbZIP47_ampCol-B_ae1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92121
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGGGGG1573017.07536826565061TruSeq Adapter, Index 6 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG13741.4915165923079428TruSeq Adapter, Index 6 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGTGGG9281.0073707406563108TruSeq Adapter, Index 6 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAAGCGGGGGGGGG6410.6958239706473008TruSeq Adapter, Index 6 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATGGGGGGGGGG6240.67736998078614TruSeq Adapter, Index 6 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGGTGG5190.5633894551730876TruSeq Adapter, Index 6 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGGGGC4170.45266551600612237TruSeq Adapter, Index 6 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGGGTG3140.3408560480237948TruSeq Adapter, Index 6 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGCATCGGGGGGGGG2880.31263229882437227TruSeq Adapter, Index 6 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGACTCGGGGGGGGG2640.2865796072556746TruSeq Adapter, Index 6 (96% over 33bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGGG2580.2800664343635002TruSeq Adapter, Index 6 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATAGGGGGGGGG2430.2637835021330641TruSeq Adapter, Index 6 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGGGGT2140.23230316648755442TruSeq Adapter, Index 6 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTGGGG1910.20733600373421912TruSeq Adapter, Index 6 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATGGGGGGG1430.15523062059682374TruSeq Adapter, Index 6 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGGGCG1050.1139805256130524TruSeq Adapter, Index 6 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT990.10746735272087798TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAAGCGGGGGG940.10203970864406595TruSeq Adapter, Index 6 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)