FastQCFastQC Report
Wed 20 Sep 2023
HWLGMBGXT_n01_AtbZIP44_ampCol-B_ae2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLGMBGXT_n01_AtbZIP44_ampCol-B_ae2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8294579
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATGCC2510273.0263983259427634TruSeq Adapter, Index 19 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATGGC318210.38363610739014004TruSeq Adapter, Index 19 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT288390.347684915653947TruSeq Adapter, Index 19 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTTTGCC264240.3185695138957625TruSeq Adapter, Index 19 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCGCGTATGCC198840.23972283584254248TruSeq Adapter, Index 19 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTTTGGC173730.20945005165421895TruSeq Adapter, Index 19 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATTCC172670.20817210855427382TruSeq Adapter, Index 19 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTAGCTCGTATGCC123100.1484101845313668TruSeq Adapter, Index 19 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)