FastQCFastQC Report
Wed 20 Sep 2023
HWLGMBGXT_n01_AtbZIP44_ampCol-B_ae1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLGMBGXT_n01_AtbZIP44_ampCol-B_ae1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6371366
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC2083073.2694244844826055TruSeq Adapter, Index 27 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT212580.33364901655312224TruSeq Adapter, Index 27 (97% over 39bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCGCGTATGCC188560.29594909474671527TruSeq Adapter, Index 27 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGGC169800.26650485939749813TruSeq Adapter, Index 27 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGAGCTCGTATGCC160310.25161009428747305TruSeq Adapter, Index 27 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATTCC120540.18919019877370097TruSeq Adapter, Index 27 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTTTGCC115550.18135828329435164TruSeq Adapter, Index 27 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGGATGCC73640.1155796104006582TruSeq Adapter, Index 27 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGCTCTCGTATGCC73130.11477915410918162TruSeq Adapter, Index 27 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATGTCGTATGCC67250.10555036392509863TruSeq Adapter, Index 27 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)