FastQCFastQC Report
Wed 20 Sep 2023
HWLGMBGXT_n01_AtbZIP39_ampCol-B_ae2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLGMBGXT_n01_AtbZIP39_ampCol-B_ae2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6958955
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATGCC2353823.3824331383088406TruSeq Adapter, Index 1 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT194200.27906488833452725TruSeq Adapter, Index 1 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGCTCTCGTATGCC123950.17811582342463775TruSeq Adapter, Index 1 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATGTCGTATGCC94090.13520708209781498TruSeq Adapter, Index 1 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGAGCTCGTATGCC89340.12838134461280465TruSeq Adapter, Index 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)