FastQCFastQC Report
Wed 20 Sep 2023
HWLGMBGXT_n01_AtbZIP26_ampCol-B_ae1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWLGMBGXT_n01_AtbZIP26_ampCol-B_ae1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGGAT11.2195121951219512TruSeq Adapter, Index 7 (97% over 35bp)
GTTCGAGGAATGGTCGACCAGAAGTTGAAATCGTCGACCGGGTCCGAGAA11.2195121951219512No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATGTCGTTT11.2195121951219512TruSeq Adapter, Index 7 (97% over 35bp)
ACACTTAAACCCATTTTCTCCCAAAAGATGCTAAAACCATCAAAAATTTG11.2195121951219512No Hit
AGATTAAAATTGTCAAAATAATTTTAGCACCTAAATAAATTCTTACATTA11.2195121951219512No Hit
CTAATAGATGGGCAGCCGATAAGGGCACTGCCCAGCTTATAGCAGCAGAA11.2195121951219512No Hit
GAAACATTCCTACCCCTATACACTATCTGAATGTCGTCTCAATCTCGCAT11.2195121951219512No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATGCC11.2195121951219512TruSeq Adapter, Index 7 (96% over 32bp)
ATTATCATGATTGTCAGCTTTTCGCTTCTTTCTCAACGTATTGTATAGTC11.2195121951219512No Hit
AACTAGTTCAGGAAGAATTTGCAAATTAATAAAACCCGGTGGTCGTATTT11.2195121951219512No Hit
ATTTTCGAATTGAGAGAAATAGTGAGAGAGATCAAGAATGCTCACTATTT11.2195121951219512No Hit
TAAAAATACGATAAATTATTCTGTCAATATAACAAATCAATACAGATATT11.2195121951219512No Hit
TCTCAGTCCGGAGGTATATACACCCCCCACTAATAAATTCTAGTTCATTG11.2195121951219512No Hit
GAAGCATTAGTAGGTGCCAAGACAACATACAGAGTCATGTTAGGGAATCA11.2195121951219512No Hit
AACTCCTATGTTCCTTTCATTACAGTATTTCTGAACAAGTTCCTGGATAA11.2195121951219512No Hit
CTATATGTAAAATTAAAAAAGCGATAGATATAATAGATATATATCTAAAT11.2195121951219512No Hit
TCCTCAAATTCTACTAATTCACCTGCCATTACTTCATCAAGACCATAAAT11.2195121951219512No Hit
GATCTCTCCGCCATTATTTTTCGTTAGCTTCTTCTTCTTTATGGACTAAG11.2195121951219512No Hit
GACTTTGGCTAAACCATCAAAGCTTTGAGAAGCAGGAAGAAGTTTGTTAG11.2195121951219512No Hit
CGTGGATTGAAACATCCGTAAGTAGGAAAATCCTGAGAAACAGTAACATT11.2195121951219512No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGGGTTTGGC11.2195121951219512TruSeq Adapter, Index 7 (96% over 32bp)
CATCTGTAAGTCCTTCTGTGATGTATCACTCAGTTTGCAAGCCATCTGTA11.2195121951219512No Hit
TATCGAAGGCGCATTCTTCGATTGCTGGGATACTCAGCGCCATGTTGTTA11.2195121951219512No Hit
GGTAATCTCCTTGTACTGACTTCCATTCCCTAATCTTTTTTTCACTTGGT11.2195121951219512No Hit
ATTCGGTGAATTTCTCATATAATAGATGTAGAAAAGTGGATCAGAAGTTG11.2195121951219512No Hit
AATCACTTTACTAAATCAAAATTGCTACCTGACTAGAGCTTAATTCGTCT11.2195121951219512No Hit
AGTGAGTTTAGTATTTTGCGAAAGACCCATTAACAGATTATGATTTGTGC11.2195121951219512No Hit
ACATTGCACATTTTATTGACTCATGTTTCGGTTGATTAAAAAAACTAGGT11.2195121951219512No Hit
ACGAGATTCAGCATTTTTGAACCACGAAACTCTTGGTTTCCATTTTGGTT11.2195121951219512No Hit
AGATAATCATCAATCACGGACTTGCTCAATTAGTTTTCGTGGATAACTGA11.2195121951219512No Hit
TCTTCCTTCTAAAACAAGCATTTGCACACTCATCACAAAAAATCAGTTGG11.2195121951219512No Hit
CAAGAGTATGACATGAGAAAACACATTTGTATAAGAAAAAAGGGCAAAAG11.2195121951219512No Hit
AAACTTAAAAAGAGAGCTAAAAAAGACCTCTTAAAAGCTAAGAAAGAAAA11.2195121951219512No Hit
ATTGATAATACACAGCATATAGCTACCTTAACAGAACCAATAGATTTGTG11.2195121951219512No Hit
TAGAAAATCATATTCTTTCTTAGAAGAAAACAGATTTTTTTGTCTCATTC11.2195121951219512No Hit
CGGAAGAGCACACGTCTGAACTCCATTCACGAATTCGTATCTAGTATACC11.2195121951219512TruSeq Adapter, Index 9 (96% over 32bp)
TCATATCCAAGAGAGCTTCCATACACACACGATGTACTACGACTCTTATA11.2195121951219512No Hit
AAAAGAACAAAAAATTATGAAAACCTTTTGCCTCTCTTTAGATTTGCTAT11.2195121951219512No Hit
CATATTCGACTCCAAAACACTAACCAACCTTCTTCTTGCTTCTCAAAGCT11.2195121951219512No Hit
AAATAAAACTAATACTGATCTCCATAAAATAATGTAAGAAACATGTTATG11.2195121951219512No Hit
CACATACACATAGGAAGATATGATGGGTTACGAGCAGATGAATCAGATGA11.2195121951219512No Hit
CTAATGAAACATAAATCGGAATCCTTTGAAAAATTCAAGGAGTTCCAGAA11.2195121951219512No Hit
TTCTAGTAGAATCGAAAAGATGTAAACACAAGCAAACAGATCGGAAGAGC11.2195121951219512No Hit
TGTATAAAGAATTCGAATCAGTAGCGACGAGTAATTTCAAATGGGTTATA11.2195121951219512No Hit
CTAAAATGCAAAATCGTGGAAAATTAGAGAAATTCGAGTTTATCTTTTCC11.2195121951219512No Hit
ATATAAAGAAGATGAGATTACGATCATGAAGAATCTGGGTCGCACCGACT11.2195121951219512No Hit
AAATAACAGAGTATGCAACTTTTTTTGTTTTTTCATTTATTTGGTACTAT11.2195121951219512No Hit
TCCAAAATACTAACCAACCTTCTTCTTGCTTCTCAAAGCTTTCATGGTGT11.2195121951219512No Hit
ACGATGCTCCGGTCGAAGGAATTCGGAGAATACTCGAGGAGTATACAGGG11.2195121951219512No Hit
AAGTCTGCTACATGCTCGATGTAGCATCCCCGAAAAATCTCGGGATAAAC11.2195121951219512No Hit
GATGAACATTTATTCCATGTAAATCAACTAACCTTGAGAAAAAGACCTTC11.2195121951219512No Hit
GCTCGACCTCGTGGTCGAGTGGTGAGGTGAAGTGGTGATGCCATCTACTC11.2195121951219512No Hit
ATGAATAAATCTCATGAATAACAAATAGATGACTTTTAACATAAAATTTA11.2195121951219512No Hit
CTTCATGGTATCTTCCTCTATTTCGCCAAGCCTTGGTATGTTCGTACGGT11.2195121951219512No Hit
CTGTCACTTGGTTAGTGTTGCCTACACTCATCAGCTCCTCCAGTGTACCA11.2195121951219512No Hit
ATACACAAGCACCGTCTTCAGAAACCAGAGTACAATTTAACCACACAAAA11.2195121951219512No Hit
GATGTGCCATATCGGTTAGCCATAAACTTTGCGTTTGTGGATGGAATCCC11.2195121951219512No Hit
AAGGGTTATGGGAACGTACGGATACTGTGCTCCTGACTACGCTATGACGG11.2195121951219512No Hit
AAGGTTTGTGCATGTTAGATACACATCGAAATTCTTTGGTTGGCTATCCT11.2195121951219512No Hit
ACTGTATTTACTATTCACCTTTGCCACTTATGGATTGATTCCTCTCGTCT11.2195121951219512No Hit
TCGCTGCTTTCGACGCTGCCATACACGACGGAGCTGACGTCATATCCGTC11.2195121951219512No Hit
AGCAGATCCCACCCATTCCTCATGTAAGATGATCTGCTTTTCACGCCTCT11.2195121951219512No Hit
CATATTCGACTCCAAAAAACTAACCAACCTTCTTCTTGCTTCTCAAAGGT11.2195121951219512No Hit
ATAATAAAGATCCGGCCCATTACTGTGATCAGAAACAACATTTAAAAAGT11.2195121951219512No Hit
TTATCCTGGAATGTAATTCACCTGATCGAGTATTCTGGCATCTGAGCCAA11.2195121951219512No Hit
CTTAAAGGCGTAAGAATTGTATCCTTGTTAGAAGACACAAAGCCAGATCG11.2195121951219512No Hit
AAACTGGGTTGAGCGTTGGAGTTTACTGCACTGGAATGAACTGCAGATCT11.2195121951219512No Hit
TAATACTTCTCAAAAATCGAAATTCTTGATCAATGGAGGAACAATATCAC11.2195121951219512No Hit
ATATACAACAGATATTACTGTCAAGAGTGATTTTATTAATATTTTAATTT11.2195121951219512No Hit
TGGAAAACACCTCCAAAACACTATTGCTTCTGAAATCAGAGTCAGGTGTT11.2195121951219512No Hit
AACAAGTAGTTTTGTTGGTTGGTTAATTGGTCACATTTTATTCATGAAAT11.2195121951219512No Hit
ATGATTGTATCATTAACTATTTCTTTATTTTGGTGTGAGGAACTTATCAT11.2195121951219512No Hit
TCTACTTGAGTTTAGGGTTTATCATCTTTTGGACATCGAATTTTGGTGGA11.2195121951219512No Hit
GTTCAGCTAGTCCAACGCTCTCGCTATGGCAAGGAAACTACTCAGGGTGA11.2195121951219512No Hit
TGATTGGTAATACGATGTCTGAAGTTTGGTTTATCTGGTTAGAGATCCTT11.2195121951219512No Hit
TAAATCATATAAAACAGAATATCTTAAATATGTAAACTTGATTAGAGAGT11.2195121951219512No Hit
TAGTATTCTCTTATTTATTACCTGTGTCTACTATTTAGGCAGAATACCAT11.2195121951219512No Hit
AACACTAAACTATAGACTTTAAACTTTAAAACCTAAATCCTAGATCCTAA11.2195121951219512No Hit
TTCCAAATTCGGCCGAAAACTTTTCCTTATTTCTAAAGAAGGCCCATCAC11.2195121951219512No Hit
ATCAAATTATTTCGTGGATTGGATTTCACTTGACTGTCTACGGCTCCATT11.2195121951219512No Hit
TGTCATCATTATGATGATTCAATCCATATTAATCCCTATTTCAGAACTAT11.2195121951219512No Hit
AGGTATAATTTTATGTTACAAAATCATAAAAGCTACAGACCAAAAAAAGG11.2195121951219512No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)