FastQCFastQC Report
Wed 8 Nov 2023
HWL5VBGXT_n01_AtbZIP69_ampCol-B_af1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWL5VBGXT_n01_AtbZIP69_ampCol-B_af1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5034579
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATGCC556401.1051569555269667TruSeq Adapter, Index 7 (96% over 32bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGCGTATGCC105040.20863710749200676TruSeq Adapter, Index 7 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT62060.12326750657800781TruSeq Adapter, Index 7 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATGGC54520.10829108054516574TruSeq Adapter, Index 7 (96% over 32bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)