FastQCFastQC Report
Wed 8 Nov 2023
HWL5VBGXT_n01_AtbZIP66_ampCol-B_af2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWL5VBGXT_n01_AtbZIP66_ampCol-B_af2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5244929
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATGCC673841.2847457038979937TruSeq Adapter, Index 7 (96% over 32bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGCGTATGCC126510.24120440905873083TruSeq Adapter, Index 7 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT67460.12861947225596382TruSeq Adapter, Index 7 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATGGC66840.1274373780846223TruSeq Adapter, Index 7 (96% over 32bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATTCC55550.10591182454519404TruSeq Adapter, Index 7 (96% over 32bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)