FastQCFastQC Report
Wed 8 Nov 2023
HWL5VBGXT_n01_AtbZIP54_ampCol-B_af2wg.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWL5VBGXT_n01_AtbZIP54_ampCol-B_af2wg.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4936451
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATGCC626311.2687455015759297TruSeq Adapter, Index 13 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCGCGTATGCC88370.1790152479990179TruSeq Adapter, Index 13 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT72790.14745411227620817TruSeq Adapter, Index 13 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATGGC54910.1112337588279515TruSeq Adapter, Index 13 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATTCC51410.10414364489792363TruSeq Adapter, Index 13 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)