FastQCFastQC Report
Wed 8 Nov 2023
HWL5VBGXT_n01_AtbZIP36_ampCol-B_af1wg.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWL5VBGXT_n01_AtbZIP36_ampCol-B_af1wg.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3659092
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATGCC79750.217950245580051TruSeq Adapter, Index 3 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGGGGTTGGC76050.20783844735251258TruSeq Adapter, Index 3 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGGGGGTGGC57000.15577635107288912TruSeq Adapter, Index 3 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGCGTATGCC54940.1501465390867461TruSeq Adapter, Index 3 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)