FastQCFastQC Report
Wed 17 Jan 2018
HWKGGAFXX_n01_ah8220spike-red1-inp_0118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKGGAFXX_n01_ah8220spike-red1-inp_0118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5582685
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT1293322.316663039379797TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA193350.074.236999
AGAGCAC197950.072.654278
GATCGGA193100.071.709211
TCGGAAG207600.069.714543
ATCGGAA213700.067.706812
CGGAAGA213850.067.589164
GAAGAGC224100.064.434836
GGAAGAG229700.062.782115
AAGAGCA235950.061.1324357
GCCGTCT161250.042.51296250-51
TGCCGTC166300.042.14116750-51
AGGGGGG145550.041.84535274-75
CCGTATC161900.041.5119238-39
CGTATGC169950.041.3163546-47
TATGCCG170550.041.29768448-49
GTATGCC170100.041.2937446-47
GTTCCGT170750.041.07291834-35
TATCTCG161100.041.04664240-41
AGTTCCG172700.041.03088834-35
ATGCCGT171950.040.9204148-49
Produced by FastQC (version 0.11.2)