FastQCFastQC Report
Wed 17 Jan 2018
HWKGGAFXX_n01_ah8220spike-red1-chip_0118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKGGAFXX_n01_ah8220spike-red1-chip_0118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5870464
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT223140.3801062403244445TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG31950.033.0923774-75
GCCGTCT38850.030.63511750-51
TGCCGTC40450.030.12113450-51
GAGCACA84700.029.1763959
TATGCCG44300.028.14040448-49
AAGGGGG37250.027.31164474-75
GTATGCC45150.027.24479946-47
CGTATGC45300.027.20650546-47
AGAGCAC91700.027.0518868
ATGCCGT46050.026.40700348-49
CTCGTAT44600.026.36369744-45
CCGTCTT49650.024.25480752-53
TCGTATG50250.024.00780544-45
AAAGGGG45100.023.44418572-73
ATCTCGT47900.023.31526842-43
TCGGAAG108650.022.9639113
GATCGGA104000.022.9558221
CACAGTC53350.022.89908630-31
TCTCGTA49850.022.82771742-43
CAGTCAC53950.022.81821626-27
Produced by FastQC (version 0.11.2)