FastQCFastQC Report
Wed 17 Jan 2018
HWKGGAFXX_n01_ah8220spike-gh2ax-inp_0118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKGGAFXX_n01_ah8220spike-gh2ax-inp_0118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6206920
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG15600.08.28418774-75
TGCCGTC22300.07.91024850-51
GCCGTCT21150.07.45071750-51
CGTATGC28600.05.838526246-47
TATCTCG25050.05.724145440-41
TATGCCG28900.05.69676948-49
AAGGGGG19800.05.69623474-75
GTATGCC27050.05.651412546-47
ATGCCGT30800.05.34534548-49
CCGTCTT33050.05.265946452-53
CTCGTAT31800.05.028418544-45