FastQCFastQC Report
Wed 17 Jan 2018
HWKGGAFXX_n01_ah8220spike-gh2ax-chip_0118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKGGAFXX_n01_ah8220spike-gh2ax-chip_0118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6012785
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT62190.10342960874203883TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG16600.019.39067574-75
GCCGTCT22200.016.52916150-51
CCGTACG22150.016.12229332-33
TATGCCG24950.014.61284948-49
TGCCGTC24700.014.47524250-51
AAGGGGG20600.014.370893574-75
CGTACGT25750.013.86789934-35
GTATGCC26150.013.85169546-47
ATGCCGT28200.012.6785148-49
CGTATGC30350.012.6323146-47
GTACGTA29100.012.27142334-35
CCGTCTT33250.011.38906652-53
AAAGGGG27750.011.17636172-73
ACCGTAC34300.010.959332-33
CTCGTAT34300.010.62702944-45
TCTCGTA32750.010.33788142-43
ATCTCGT33850.010.14085442-43
CGTAATC34200.010.09964238-39
GAGCACA72650.010.0239699
TCGTATG39450.09.89544744-45
Produced by FastQC (version 0.11.2)