FastQCFastQC Report
Wed 17 Jan 2018
HWKGGAFXX_n01_ah8219spike-red1-inp_0118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKGGAFXX_n01_ah8219spike-red1-inp_0118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5734694
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC142120.24782490574039345TruSeq Adapter, Index 12 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG73430.12804519299547631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT31200.025.92987448-49
AGGGGGG28850.025.2476172-73
TGCCGTC33350.024.32875348-49
AAGGGGG31250.022.48151672-73
GTATGCC36800.022.17264744-45
TATGCCG38250.021.64185746-47
ATGCCGT39050.021.13826846-47
CGTATGC39300.020.58267444-45
CCGTCTT43400.019.12919650-51
CTCGTAT40100.018.8842-43
ATCTCGT39150.018.73381240-41
TCGTATG44400.018.48131842-43
AAAGGGG41000.017.42564470-71
TCTCGTA42900.017.37021640-41
GAGCACA97650.016.8401859
AATCTCG46050.016.2273438-39
CTGCTTG49950.016.14618556-57
AGAGCAC104250.015.86418158
GTCACCT53700.015.48719528-29
CAGTCAC54300.015.0995426-27
Produced by FastQC (version 0.11.2)