FastQCFastQC Report
Wed 17 Jan 2018
HWKGGAFXX_n01_ah8219spike-red1-chip_0118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKGGAFXX_n01_ah8219spike-red1-chip_0118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5404973
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC587641.0872209722416746TruSeq Adapter, Index 7 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG62590.11580076348207476No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA108800.065.250569
GATCGGA108300.063.367991
AGAGCAC112750.062.9229438
TCGGAAG116850.061.1262553
CGGAAGA125550.056.8530774
ATCGGAA128200.055.7914472
GAAGAGC136900.052.0708966
AAGAGCA146000.048.8825157
GGAAGAG146550.048.866595
TGCCGTC84900.039.61351848-49
GCCGTCT81550.039.45274748-49
AGGGGGG80000.038.62235672-73
CATCTCG79450.038.27256438-39
TATGCCG89850.038.13717746-47
GTATGCC89900.038.03149444-45
CGTATGC90400.037.89917844-45
ATGCCGT88950.037.8091746-47
AAGGGGG81450.036.72318672-73
CTCGTAT85850.036.4490342-43
CAGTCAC97800.036.0579226-27
Produced by FastQC (version 0.11.2)