FastQCFastQC Report
Wed 17 Jan 2018
HWKGGAFXX_n01_ah8219spike-gh2ax-inp_0118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKGGAFXX_n01_ah8219spike-gh2ax-inp_0118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6654392
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG16350.07.760059474-75
AAGGGGG21350.07.15326874-75
GCCGTCT19100.06.403313650-51
TGCCGTC21650.06.300932450-51
CGTTTCG26150.05.929934532-33
AGTCACG27050.05.819361728-29
CTCGTAT26100.05.40564544-45
GTCACGT30050.05.316579328-29
GTATGCC25250.05.30913546-47
GTTTCGG28100.05.267370734-35
CGTATGC26400.05.256036346-47
GAGCACA63050.05.21631059
ATGCCGT26550.05.04940548-49
TATGCCG26800.05.00230248-49
Produced by FastQC (version 0.11.2)