FastQCFastQC Report
Wed 17 Jan 2018
HWKGGAFXX_n01_ah8219spike-gh2ax-chip_0118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKGGAFXX_n01_ah8219spike-gh2ax-chip_0118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7156683
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT117680.16443371880520627TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG25550.024.64569774-75
GCCGTCT32200.021.98494150-51
TGCCGTC37600.019.4530350-51
GTATGCC39050.019.15162546-47
TATGCCG39050.018.73070148-49
CACGTGG40300.017.8394630-31
ATGCCGT41950.017.66010548-49
CGTATGC42350.017.6592946-47
AGTCACG42800.017.5656428-29
ACGTGGC40800.017.5056732-33
GTCACGT42800.017.45585428-29
CGTGGCC41050.017.34182732-33
TCACGTG43150.017.20567130-31
GAGCACA88300.017.0270989
AAGGGGG31200.016.94435974-75
CTCGTAT43150.016.72972344-45
GGCCTTA42950.016.52071436-37
TATCTCG41100.016.07120340-41
AGAGCAC95450.015.6530698
CCGTCTT46950.015.62810652-53
Produced by FastQC (version 0.11.2)