FastQCFastQC Report
Wed 17 Jan 2018
HWKGGAFXX_n01_ah8218spike-red1-inp_0118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKGGAFXX_n01_ah8218spike-red1-inp_0118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6297478
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC946521.5030143813126462TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA183350.054.915589
AGAGCAC189300.053.3636678
GATCGGA204750.047.753771
TCGGAAG211400.047.3916783
CGGAAGA217350.046.2456674
ATCGGAA221750.045.2447242
GAAGAGC223400.045.161546
AAGAGCA232800.043.513467
GGAAGAG234900.043.07065
GCCGTCT122050.039.92553348-49
TGCCGTC124000.039.90458348-49
AGGGGGG107500.039.6491872-73
GTATGCC126600.038.92778444-45
GTCACGC126850.038.87624428-29
CGTATGC129100.038.4653644-45
TATCTCG125700.038.45346538-39
TATGCCG129100.038.3802446-47
TCACGCC129400.038.2750630-31
CTCGTAT128300.037.912242-43
ATGCCGT131400.037.58316446-47
Produced by FastQC (version 0.11.2)