FastQCFastQC Report
Wed 17 Jan 2018
HWKGGAFXX_n01_ah8218spike-red1-chip_0118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKGGAFXX_n01_ah8218spike-red1-chip_0118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5583616
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC532550.9537726090046307TruSeq Adapter, Index 5 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG88180.15792633304295997No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC62420.11179135527944616TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA130200.055.3200879
AGAGCAC137100.052.604478
GATCGGA145300.048.0357131
TCGGAAG152500.047.9123273
CGGAAGA154850.047.124524
ATCGGAA160750.045.571932
GAAGAGC164800.043.9656876
AAGAGCA176950.041.129427
GGAAGAG178900.040.710655
TGCCGTC89200.039.41884648-49
AGGGGGG80150.039.31196672-73
GCCGTCT86750.039.0680848-49
GTATGCC93500.037.6743744-45
TATGCCG94300.037.6101946-47
CGTATGC94650.037.39053744-45
ATGCCGT94100.037.36521546-47
GTCACAC96950.036.93090428-29
GATCTCG75600.036.92722338-39
CACACAG97500.036.7714330-31
CTCGTAT85300.035.9687742-43
Produced by FastQC (version 0.11.2)