FastQCFastQC Report
Wed 17 Jan 2018
HWKGGAFXX_n01_ah8218spike-gh2ax-inp_0118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKGGAFXX_n01_ah8218spike-gh2ax-inp_0118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5879933
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC59990.10202497205325299TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG18300.017.84738272-73
GCCGTCT22200.015.25720248-49
TGCCGTC23700.014.88703848-49
CGGCTAC26300.014.47270732-33
CACGGCT25900.014.33274430-31
AAGGGGG21150.013.77596872-73
GTCACGG27150.013.49897928-29
CATCTCG25500.013.45479538-39
GTATGCC27500.013.33925644-45
TCACGGC27350.013.31515930-31
ACGGCTA29650.012.75826632-33
CTCGTAT29250.012.61915642-43
TATGCCG29500.012.43796946-47
AGTCACG30600.012.28413928-29
CGTATGC30100.012.2651544-45
GGCTACA31300.011.93506634-35
GAGCACA61900.011.8401839
ATGCCGT30600.011.7602646-47
AGAGCAC68300.010.9370678
ATCTCGT33500.010.59551740-41
Produced by FastQC (version 0.11.2)