FastQCFastQC Report
Wed 17 Jan 2018
HWKGGAFXX_n01_ah8218spike-gh2ax-chip_0118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKGGAFXX_n01_ah8218spike-gh2ax-chip_0118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5407794
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG13600.017.10403472-73
GCCGTCT16100.015.19082848-49
AAGGGGG18400.012.8975172-73
TGCCGTC21250.011.39862148-49
TATGCCG23350.011.27937546-47
GTATGCC24300.011.03072944-45
AAAGGGG24700.010.84707570-71
CGTATGC26650.010.67563644-45
ATGCCGT24750.010.45132446-47
TATCTCG24800.010.23327338-39
CTCGTAT27550.09.81335742-43
CCGTCTT28100.08.70388350-51
TAGCTTA32750.08.68061134-35
GAGCACA63950.08.5960579
TCTCGTA29200.08.45258540-41
TCGTATG33900.08.39125642-43
ATCTCGT32350.08.35615740-41
AGAGCAC67850.08.240538
ACTAGCT38400.07.831933532-33
GCTTATC34600.07.673555436-37
Produced by FastQC (version 0.11.2)