FastQCFastQC Report
Wed 17 Jan 2018
HWKGGAFXX_n01_ah8151spike-red1-inp_0118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKGGAFXX_n01_ah8151spike-red1-inp_0118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5831039
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT605061.037653838363969TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA187050.043.654389
AGAGCAC195000.041.9465458
GCCGTCT98600.039.6447150-51
GTCCCGA93850.039.55498536-37
CGTCCCG101800.039.11845434-35
CCCGATC83050.039.10216538-39
TGCCGTC101900.039.00710350-51
AGGGGGG87500.038.53404274-75
CGATCTC84500.037.97472440-41
TATGCCG105800.037.76929548-49
GATCTCG84900.037.6850540-41
GTATGCC105300.037.63415546-47
CGTATGC107350.037.37555746-47
TCGGAAG220300.037.1964263
TCCCGAT91600.037.1921236-37
CCGATCT85500.037.10223438-39
CGGAAGA223200.036.692094
ATGCCGT108000.036.62970448-49
CCGTCCC109500.036.6251334-35
CCCGTCC109650.036.61960632-33
Produced by FastQC (version 0.11.2)