FastQCFastQC Report
Wed 17 Jan 2018
HWKGGAFXX_n01_ah8151spike-red1-chip_0118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKGGAFXX_n01_ah8151spike-red1-chip_0118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5515194
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT865991.5701895527156433TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA152550.069.75949
AGAGCAC158800.067.132188
GATCGGA155950.065.699731
TCGGAAG164200.065.301143
CGGAAGA169450.063.056674
ATCGGAA173200.061.9101332
GAAGAGC185850.057.4417886
GGAAGAG196350.054.585415
AAGAGCA199050.053.7928967
TGCCGTC115300.041.0037250-51
GCCGTCT109300.040.2418550-51
GTATGCC121200.040.03422546-47
AGGGGGG115150.039.35882674-75
ATGCCGT121850.039.24373248-49
CGTATGC124250.039.18400246-47
TCACATG135850.039.0331830-31
CAGTCAC136000.038.55807526-27
TATGCCG126700.038.48408548-49
CACATGT137050.038.4685530-31
CCAGTCA137900.038.3164526-27
Produced by FastQC (version 0.11.2)