FastQCFastQC Report
Wed 17 Jan 2018
HWKGGAFXX_n01_ah8115spike-red1-inp_0118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKGGAFXX_n01_ah8115spike-red1-inp_0118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5545155
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT1567732.827206813876258TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGCTCTCGTAT83600.15076224199323554TruSeq Adapter, Index 19 (97% over 44bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG81710.14735386116348417No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGAGCTCGTAT68620.12374766800928017TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA254650.073.134789
AGAGCAC262500.071.108798
GATCGGA266850.067.267381
TCGGAAG279350.066.946393
CGGAAGA283400.065.906174
GAAGAGC288850.064.760276
ATCGGAA288950.064.742532
AAGAGCA298650.062.611577
GGAAGAG300450.062.2130365
TGCCGTC210950.043.63953850-51
GCCGTCT208450.043.49720850-51
CGATCTC180500.043.47114640-41
GATCTCG181950.042.9955240-41
GTATGCC213900.042.748946-47
TATGCCG215450.042.71656448-49
CGTATGC216550.042.61674546-47
CCGTCTT215500.042.3338652-53
AGTCACG219550.042.3021128-29
ATGCCGT216450.042.236748-49
AGGGGGG193800.041.93467774-75
Produced by FastQC (version 0.11.2)