FastQCFastQC Report
Wed 17 Jan 2018
HWKGGAFXX_n01_ah8115spike-red1-chip_0118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKGGAFXX_n01_ah8115spike-red1-chip_0118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6174021
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT185390.3002743268932839TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC39000.027.25995350-51
ACGTCCG39400.027.19266532-33
CCGCACA37800.026.54155236-37
CGTCCGC40600.026.273232-33
GTATGCC41200.026.14654246-47
GCCGTCT39700.026.12759250-51
CATCTCG36850.025.90035640-41
ATGCCGT42000.025.3693348-49
AGGGGGG37150.025.11029474-75
CGTATGC46100.023.41842846-47
CTCGTAT45900.022.95326444-45
AGTCACG48200.022.66426328-29
GTCCGCA46650.022.4621134-35
CACGTCC48850.022.17149230-31
TCACGTC49400.021.92464330-31
GTCACGT50200.021.901728-29
AAAGGGG40900.021.88914572-73
CGCACAT44800.021.55529836-37
TCGTATG49700.021.48219144-45
GAGCACA100250.021.4636129
Produced by FastQC (version 0.11.2)