FastQCFastQC Report
Wed 17 Jan 2018
HWKGGAFXX_n01_ah7011spike-red1-inp_0118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKGGAFXX_n01_ah7011spike-red1-inp_0118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6631561
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC119440.18010842394422671TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG28950.022.23764472-73
GCCGTCT32000.021.3168548-49
TGCCGTC35100.019.90326748-49
AAGGGGG32600.018.81090472-73
GTATGCC37900.018.79933244-45
TATGCCG40450.017.67647646-47
CGTATGC40450.017.49794244-45
ATGCCGT43150.016.24336846-47
CCGTCTT43700.016.04050650-51
CTCGTAT42050.015.99043842-43
AAAGGGG44000.014.95637970-71
TCGTATG46600.014.68139742-43
ATCTCGT44700.014.67163140-41
ACTGACC47050.014.63048632-33
TCTCGTA45700.014.60776740-41
CTGCTTG50250.013.94465356-57
CTGACCA53850.012.564857532-33
AATCTCG53150.012.29076938-39
CACTGAC58750.011.75660830-31
GAGCACA122900.011.6974979
Produced by FastQC (version 0.11.2)