FastQCFastQC Report
Wed 17 Jan 2018
HWKGGAFXX_n01_ah7011spike-red1-chip_0118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKGGAFXX_n01_ah7011spike-red1-chip_0118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6315091
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC489760.7755391014951328TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA129000.042.321829
AGAGCAC135250.040.296628
GCCGTCT69500.036.68095448-49
AGGGGGG62900.036.53371472-73
TGCCGTC74350.035.3952748-49
TCGGAAG156300.034.993993
GTATGCC76850.034.542644-45
GATCGGA152150.034.350421
CGGAAGA160500.034.2246484
TATGCCG77450.034.1894846-47
ATGCCGT77900.033.96179246-47
CGTATGC78400.033.8296944-45
TATCTCG74400.033.25663438-39
AAGGGGG65800.032.78101772-73
ATCGGAA168450.032.58412
GAAGAGC168600.032.552796
CTCGTAT78250.032.35886442-43
CCGATGT85400.031.69456332-33
CCGTCTT81200.031.36717850-51
AAGAGCA176400.031.2956377
Produced by FastQC (version 0.11.2)