FastQCFastQC Report
Wed 17 Jan 2018
HWKGGAFXX_n01_ah7011spike-gh2ax-inp_0118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKGGAFXX_n01_ah7011spike-gh2ax-inp_0118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5847964
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC739011.2637047697284047TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA177050.048.6138539
AGAGCAC185100.046.6261528
TCGGAAG206000.041.8792723
GATCGGA202350.040.9274981
CGGAAGA214050.040.457944
AGGGGGG93850.040.2582172-73
TGCCGTC106800.039.96823548-49
GAAGAGC217100.039.8895536
GCCGTCT106000.039.6042648-49
ATCGGAA219350.039.503832
GTATGCC110250.038.5830944-45
AAGAGCA225600.038.4640127
GATCTCG99450.038.13923638-39
GGAAGAG228550.037.788355
CGTATGC114550.037.58636544-45
CGATCAG113650.037.31524732-33
TATGCCG116000.037.101546-47
CTCGTAT111000.036.66578342-43
ATGCCGT115800.036.61723746-47
CACGATC117450.036.26704430-31
Produced by FastQC (version 0.11.2)