FastQCFastQC Report
Wed 17 Jan 2018
HWKGGAFXX_n01_ah7011spike-gh2ax-chip_0118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKGGAFXX_n01_ah7011spike-gh2ax-chip_0118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6236565
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG15700.015.56444672-73
AAGGGGG19550.012.98006772-73
GCCGTCT20550.011.78842448-49
GTATGCC25100.010.86735744-45
TGCCGTC25550.010.49407748-49
TATGCCG26000.010.49294746-47
ATGCCGT29300.09.87302746-47
GTCACAC30750.09.47387928-29
AAAGGGG27500.09.31536870-71
GAGCACA64200.09.2210839
CGTATGC29100.09.21194844-45
CTCGTAT29450.08.54237942-43
AGAGCAC71700.08.32206258
TCTCGTA31750.07.995881640-41
CCGTCTT33050.07.68559250-51
CAGTCAC41400.07.6043626-27
ATCTCGT32600.07.57108340-41
ACACTTG43100.07.52273332-33
AGTCACA43200.07.504928628-29
TCGTATG34300.07.40303942-43
Produced by FastQC (version 0.11.2)