FastQCFastQC Report
Wed 17 Jan 2018
HWKGGAFXX_n01_ah4206spike-gh2ax-chip_0118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKGGAFXX_n01_ah4206spike-gh2ax-chip_0118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5578303
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT170610.3058457025371336TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG28650.027.8023174-75
GTATGCC34150.026.44299146-47
TGCCGTC34300.025.9170350-51
GCCGTCT34500.025.49412550-51
TATCTCG34100.025.43659240-41
AAAGGGG34550.022.85118772-73
AAGGGGG32950.022.6765874-75
ATGCCGT39350.022.5912648-49
TATGCCG41650.021.68251448-49
CGTATGC41650.021.56842646-47
CTCGTAT41150.021.54243744-45
CCGTCTT41250.021.43493852-53
GAGCACA88100.020.1587759
GTCACAC44250.020.12291528-29
ATCTCGT43850.019.9451942-43
CACACTG49150.018.45170830-31
TCTCGTA48700.018.29679942-43
AGAGCAC98100.018.1996428
TCGTATG50350.018.02648744-45
CAGTCAC51750.017.43347426-27
Produced by FastQC (version 0.11.2)