FastQCFastQC Report
Wed 17 Jan 2018
HWKGGAFXX_n01_ah119spike-gh2ax-inp_0118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKGGAFXX_n01_ah119spike-gh2ax-inp_0118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5918155
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC71610.12100054831277654TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG20700.017.70780672-73
GCCGTCT23350.017.12279148-49
TGCCGTC25350.015.67910448-49
CATCTCG26600.014.753180538-39
AAGGGGG23950.014.22567772-73
TATGCCG29300.013.88579746-47
GTATGCC30600.013.75479544-45
CTTAGGC31750.013.17200532-33
GCATCTC30500.013.09784838-39
ATGCCGT32250.012.98023646-47
CTCGTAT31850.012.91780542-43
CGTATGC31550.012.89345444-45
ACTTAGG33000.012.88665632-33
TAGGCAT33650.012.2885734-35
TCGTATG35150.011.90569642-43
TTAGGCA36850.011.73151534-35
CCGTCTT34700.011.52225550-51
AAAGGGG33550.011.41809570-71
ATCTCGT34400.011.411406540-41
GAGCACA73500.011.2506069
Produced by FastQC (version 0.11.2)