FastQCFastQC Report
Wed 17 Jan 2018
HWKGGAFXX_n01_ah119spike-gh2ax-chip_0118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKGGAFXX_n01_ah119spike-gh2ax-chip_0118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5475389
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG11500.09.39891372-73
GCCGTCT15900.07.98816448-49
AAGGGGG14150.07.804751472-73
CGATCTC17150.07.536088538-39
TGCCGTC20200.07.335664748-49
TATGCCG20350.07.050238646-47
CACGATC18150.06.86071436-37
GATCTCG19000.06.55495438-39
CGTATGC24000.06.368967544-45
CTCGTAT25050.06.28855242-43
GTATGCC22150.06.157737744-45
CCGTCTT28350.06.05658850-51
ATGCCGT23950.05.794085546-47
ATCTCGT26300.05.631250440-41
TCTCGTA26250.05.28375640-41
AAAGGGG21900.05.151430670-71
CAGTCAC36253.6379788E-125.05481394