FastQCFastQC Report
Tue 23 Jan 2018
HWKFWAFXX_n01_vs9_180118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKFWAFXX_n01_vs9_180118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7245978
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT2245113.0984223247710663TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGTAT142070.19606739076491816TruSeq Adapter, Index 15 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG88850.12261974850047846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG348450.042.9552311
GATCGGA318050.042.7870061
ACGTCTG350200.042.665215
ACACGTC351700.042.65211513
CACACGT351850.042.54640212
CACGTCT351750.042.5272214
AGCACAC353150.042.458310
CGTCTGA351300.042.43767516
GAGCACA354550.042.3523989
TCGGAAG356400.042.2229423
GAACTCC350100.042.04903821
AGAGCAC357750.042.035068
ATCGGAA360150.041.846142
CAGTCAC352450.041.7539827
CTCGTAT294950.041.744244
TCCAGTC351800.041.71230325
GTCTGAA357750.041.6661217
AGTCACA354450.041.5804528
GTCACAT354000.041.57087329
CCAGTCA353100.041.55250526
Produced by FastQC (version 0.11.2)