Basic Statistics
Measure | Value |
---|---|
Filename | HWKFWAFXX_n01_vs8_180118.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 8418829 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 38 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT | 422510 | 5.018631451001084 | TruSeq Adapter, Index 14 (97% over 44bp) |
AATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT | 25109 | 0.29824812928258787 | TruSeq Adapter, Index 14 (97% over 41bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTAGCTCGTAT | 16401 | 0.19481331667385096 | TruSeq Adapter, Index 14 (97% over 41bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 10026 | 0.11909019651070239 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGTAT | 9826 | 0.11671456921146633 | TruSeq Adapter, Index 14 (97% over 45bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GATCGGA | 52225 | 0.0 | 43.46482 | 1 |
GCACACG | 57465 | 0.0 | 43.29219 | 11 |
CACACGT | 57730 | 0.0 | 43.11201 | 12 |
ACACGTC | 57770 | 0.0 | 43.074543 | 13 |
TCGGAAG | 57955 | 0.0 | 43.024273 | 3 |
CACGTCT | 57760 | 0.0 | 43.02106 | 14 |
ACGTCTG | 57735 | 0.0 | 42.986347 | 15 |
AGCACAC | 57950 | 0.0 | 42.9567 | 10 |
GAGCACA | 57975 | 0.0 | 42.949043 | 9 |
CGTCTGA | 57645 | 0.0 | 42.943302 | 16 |
AGAGCAC | 58335 | 0.0 | 42.74433 | 8 |
ATCGGAA | 58595 | 0.0 | 42.63824 | 2 |
CTCGTAT | 52865 | 0.0 | 42.60647 | 44 |
CGGAAGA | 58610 | 0.0 | 42.543705 | 4 |
TCCAGTC | 57675 | 0.0 | 42.43398 | 25 |
GAACTCC | 57950 | 0.0 | 42.417652 | 21 |
GTTCCGT | 56520 | 0.0 | 42.392258 | 35 |
CCGTATC | 52525 | 0.0 | 42.33337 | 38 |
GTCACAG | 57830 | 0.0 | 42.320248 | 29 |
CAGTCAC | 57860 | 0.0 | 42.31351 | 27 |