FastQCFastQC Report
Tue 23 Jan 2018
HWKFWAFXX_n01_vs8_180118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWKFWAFXX_n01_vs8_180118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8418829
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT4225105.018631451001084TruSeq Adapter, Index 14 (97% over 44bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT251090.29824812928258787TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTAGCTCGTAT164010.19481331667385096TruSeq Adapter, Index 14 (97% over 41bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG100260.11909019651070239No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGTAT98260.11671456921146633TruSeq Adapter, Index 14 (97% over 45bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA522250.043.464821
GCACACG574650.043.2921911
CACACGT577300.043.1120112
ACACGTC577700.043.07454313
TCGGAAG579550.043.0242733
CACGTCT577600.043.0210614
ACGTCTG577350.042.98634715
AGCACAC579500.042.956710
GAGCACA579750.042.9490439
CGTCTGA576450.042.94330216
AGAGCAC583350.042.744338
ATCGGAA585950.042.638242
CTCGTAT528650.042.6064744
CGGAAGA586100.042.5437054
TCCAGTC576750.042.4339825
GAACTCC579500.042.41765221
GTTCCGT565200.042.39225835
CCGTATC525250.042.3333738
GTCACAG578300.042.32024829
CAGTCAC578600.042.3135127